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Swamy, H. D. Narayana
- Safety Evaluation of Silk Protein Film (A Novel Wound Healing Agent) in Terms of Acute Dermal Toxicity, Acute Dermal Irritation and Skin Sensitization
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Authors
Amol R. Padol
1,
K Jayakumar
1,
N. B. Shridhar
1,
H. D. Narayana Swamy
2,
M. Narayana Swamy
3,
K. Mohan
1
Affiliations
1 Departments of Pharmacology and Toxicology, KVAFSU, Hebbal, Bangalore, IN
2 Departments of Veterinary Pathology, KVAFSU, Hebbal, Bangalore, IN
3 Departments of Veterinary Physiology, Veterinary College, KVAFSU, Hebbal, Bangalore, IN
1 Departments of Pharmacology and Toxicology, KVAFSU, Hebbal, Bangalore, IN
2 Departments of Veterinary Pathology, KVAFSU, Hebbal, Bangalore, IN
3 Departments of Veterinary Physiology, Veterinary College, KVAFSU, Hebbal, Bangalore, IN
Source
Toxicology International (Formerly Indian Journal of Toxicology), Vol 18, No 1 (2011), Pagination: 17-21Abstract
Acute dermal toxicity study was conducted in rats. The parameters studied were body weight, serum biochemistry and gross pathology. The animals were also observed for clinical signs and mortality after the application of test film. The dermal irritation potential of silk protein film was examined using Draize test. In the initial test, three test patches were applied sequentially for 3 min, 1 and 4 hours, respectively, and skin reaction was graded. The irritant or negative response was confirmed using two additional animals, each with one patch, for an exposure period of 4 hours. The responses were scored at 1, 24, 48 and 72 hours after the patch removal. Skin sensitization study was conducted according to Buehler test in guinea pigs, in which on day 0, 7 and 14, the animals were exposed to test material for 6 hours (Induction phase) and on day 28, the animals were exposed for a period of 24 hours (Challenge phase). The skin was observed and recorded at 24 and 48 hours after the patch removal. In acute dermal toxicity study, the rats dermally treated with silk film did not show any abnormal clinical signs and the body weight, biochemical parameters and gross pathological observations were not significantly different from the control group. In acute dermal irritation study, the treated rabbits showed no signs of erythema, edema and eschar, and the scoring was given as “0” for all time points of observations according to Draize scoring system. In skin sensitization study, there were no skin reactions 24 and 48 hours after the removal of challenge patch, which was scored “0” based on Magnusson/Kligman grading scale.Keywords
Draize test, erythema, irritation, sensitization- Study of Nephrotoxic Potential of Acetaminophen in Birds
Abstract Views :181 |
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Authors
Affiliations
1 Departments of Veterinary Pharmacology and Toxicology, KVAFSU, Hebbal, Bangalore, IN
2 Veterinary Pathology, Veterinary College, KVAFSU, Hebbal, Bangalore, IN
3 Veterinary Faculty Clinics, Ningarhar University, Daronta Jalalabad, Ningarhar, Afghanistan, AF
1 Departments of Veterinary Pharmacology and Toxicology, KVAFSU, Hebbal, Bangalore, IN
2 Veterinary Pathology, Veterinary College, KVAFSU, Hebbal, Bangalore, IN
3 Veterinary Faculty Clinics, Ningarhar University, Daronta Jalalabad, Ningarhar, Afghanistan, AF